MIL-DTL-44048H
down so that grips grab fabric just below stitching, not on the stitching. Run tensile machine per test
method and record force required to pull button from sewn garment.
4.4.7 Toxicity assesment. The contractor must furnish information which certifies that the finished
product is composed of materials which have been safely used commercially or provide sufficient
toxicity data to show compatibility with prolonged, direct skin contact. At a minimum, toxicity data
should include results from a primary dermal irritation study in laboratory animals (see 2.2.2) and a
repeated insult human patch test (Modified Draize Procedure) (see 2.3). The latter must be conducted
under the supervision of a qualified dermatologist using at least 100 free living individuals.
4.4.8 Gas chromatographic test method for types IX, X, XI, and XII only. (See 3.3.5)
4.4.8.1 Gas chromatograph. The gas chromatograph shall be equipped with an electron capture
detector and a GC septurn. The gas tank shall contain an argon/methane (95%/5%) mixture and be
equipped with an appropriate gas regulator. The glass column shall be 6 feet by 1/8 inch inside
diameter. The column packing shall be 3.0 percent OV-225 on 10/120 mesh Gas Chrom Q (or
equivalent).
4.4.8.2 Supplies. The following supplies are needed to perform the test method:
10 microliter syringe; analytical balance; electrical heater with variable control; heat resistant 250 ml
glass flask with a flat bottom, and single neck; Soxhlet extractor; extractor condenser; 50 ml volumetric
flask; graduated cylinder; funnel; pipettes; and boiling chips.
4.4.8.3 Reagents. The permethrin shall consist of 40 percent permethrin (weight/weight) technical
grade with a minimum of 35 percent (+) cis and a maximum 65 percent (+) trans isomers. The mixture
shall be of 80 percent 190 UV cutoff reagent grade acetonitrile and 20 percent reagent grade methanol
(volume/volume).
4.4.8.4 Preparation
4.4.8.4.1 Stock solution. Prepare a stock solution by weighing 1.000g permethrin solution (see
4.4.8.3) to the nearest 1 mg into a 1000 ml volumetric flask and dilute to volume with
acetonitrile/methanol (80/20) solution (see 4.4.8.3). The stock solution shall then contain 400mg
permethrin with a final concentration of 0.4 mg/ml.
4.4.8.4.2 Standard preparation. Place 1 ml of the stock solution into a 100 ml volumetric flask and
dilute to volume with acetonitrile/methanol (80/20) (see 4.4.8.3). One microliters (µl) of this standard
shall then contain 4 nanograms(ng) of permethrin. This amount is within the linear portion of the
permethrin concentration x peak area standard curve performed under the gas chromatographic
procedure described in 4.4.8.4.3 (the curve is linear from 0-10 ng permethrin).
4.4.8.4.3 Standard Injection. Inject 1µl of the standard solution into a gas chromatograph equipped
with an electron capture detector. Use the argon/methane (95percent/5percent) (see 4.4.8.1) carrier
gas and the 10 meter OV-225 glass capillary column (see 4.4.8.1). The following gas chromatographic
settings shall be used in the analysis:
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